AUTHOR: Jigme & Ram Chandra Bajgai
White Rust of Crucifers is caused by Albugo candida, which causes damage to quality and quantity of the produce. Conventionally, fungicides are applied to reduce disease incidences; however, improper and excessive use of chemical fungicides can be detrimental to the environmental health. In this study, different concentrations (2.5%, 5%, 7.5% and 10%) of freshly prepared aqueous extract of Ageratina adenophora were tested for antifungal activity in vitro and in vivo against A. candida using poison-food technique and Colony Forming Unit (CFU/ml). In vitro results revealed that 10% aqueous extract of A. adenophora was most effective against mycelial growth and biomass formation of A. candida.10% aqueous extract inhibited 92.7% of A. candida mycelial and total biomass formation followed by 78% at 7.5% concentration. The lowest inhibition was observed in 2.5% concentration with 2.4%. The in vivo antifungal activity of aqueous extract of A. adenophora was tested in the potted plants under normal conditions. The result revealed that the preventive control is most effective than the curative control. Preventive control of disease incidence using 10% aqueous extract led to 54.1% reduction, whereas the curative control reduced by mere 3.6%. Thus, 10% aqueous extract of A. adenophora demonstrates the potential for the control and management of white rust of crucifers.
KEYWORDS: Albugo candida, Ageratina adenophora, Aqueous extract, Antifungal activity, Curative control,Preventive control
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